Background

BORN: July 14, 1969 Pittsburgh, Pennsylvania

RESEARCH AREAS:
Molecular Regulation of Neurodegenerative Disease
Insect Molecular Genetics
Forensic Entomology

TEACHING EXPERIENCE:
Binghamton University- Cell cycle regulation, Genetics, Molecular genetics, Advanced molecular genetics, Genetics of neurodegenerative disease
During Graduate Studies- Histology (lab), General Biology, Human Anatomy and Physiology (lab), General Biology 101, 102, 113 (labs and team lecture), Insect Physiology (Graduate Level), Insect Toxicology, Introductory Entomology (lab and team lecture), Insect Physiology 631 (labs and team lecture)

EDUCATION:
Edinboro University of Pennsylvania B.S. 5/91 Biology
Duquesne University M.S. 7/93 Animal Physiology
Thesis Title: "Analysis of Diapause-related Biochemical Changes in the Corn Earworm, Helicoverpa zea" with Dr. Olushola Adeyeye.
Ohio State University Ph. D. 3/98 Entomology
Ohio State University Ph. D. 3/98 Entomology
Dissertation Title: "Isolation of Diapause-associated Clones from the Flesh Fly, Sarcophaga crassipalpis" with Dr. David Denlinger.

PROFESSIONAL EXPERIENCE:
1999 Assistant Professor Binghamton University
1998-1999 NIH Postdoctoral Fellow University of Kentucky
1993-1998 Graduate Teaching and Research Assistant Ohio State University
1991-1993 Graduate Teaching and Research Assistant Duquesne University

GRANTS & AWARDS:
2004 NSF CCLI (Co-PI; details below)
2003 NSF Equipment Grant (Co-PI; details below)
2002 NIH R15 (AREA) award (details below)
2001 USDA New Investigator Award (details below)
1998 NIH Postdoctoral Training Fellowship
1998 W. Fred Hink Research Award OSU

EXTERNAL RESEARCH SUPPORT :

Current:

New Investigator Award (#2001-2931) United States Department of Agriculture NRI. Principal Investigator. "Signal Transduction in Insect Diapause". 10/1/01 to 9/30/05. $180,000 total costs.
NIH R15 (AREA award) National Institutes of Health (NINDS). Principal Investigator. "NADPH oxidase in neuronal apoptosis". 6/1/02-5/31/05. $150,500 total costs.
NSF CCLI-A&I Introducing Flow Cytometry into the Undergraduate Biology Curriculum. Co-PI with Dr. Dennis McGee. 9/1/04 to 8/31/06. $99,928 total costs.

NSF Major Equipment Grant

Acquisition of a Laser Scanning Confocal Microscope for Multidiscipline
Research and Research Training. Co-PI with Drs. Dennis McGee and Susannah Gal. 8/1/03 to 7/31/06. $288,346 total costs.

GRANT REVIEW SERVICES:

USDA NRI grants program- 2002 and 2003

EDITORIAL SERVICES (Manuscript Reviews):

Gene: The International Journal of Genes and Genomes (2001 and 2003)
Journal of Insect Physiology (2000, 2001, 2003, 2004)
USDA internal manuscript review process (2002, 2003)

DEPARMENTAL COMMITTEES:
1999-present Graduate Committee
  Budget Committee
2001-2004 Institutional Animal Care and Use Committee (IACUC)
  Biotechnologist Faculty Search Committee
2001-2002 Biotech/ Biomedical Faculty Search Committee

GRADUATE RESEARCH SUPERVISION :
Major Advisor:  
M.S. Carli Yeager-Hall (Graduated May 2002) Thesis title: “Molecular and biochemical analysis of diapause termination and nerve cord development in two insects”
M.S. Mike Villanella (Graduated May 2003) Thesis Title: “Genetic variability in five populations of the red-backed salamander, Plethodon cinereus, demonstrated by RAPD analysis”
M.S. Eric Hillberger (Graduated August 2004) Thesis Title: “NADPH oxidase and oxidative stress in neuronal apoptosis”
M.S. Lisa DiAntonio (Expected December 2004)  
M.S. Ken Weir (Expected May 2005)  
M.S. Savvas Pavlides (Expected May 2005)  
M.S. Sherri Zola (Expected May 2006)  
Ph.D. Lina Begdache (Expected May 2008)  
   
Supervising Committee Member:  
Ph. D. John M. Baust
Ph. D. Gongchu Li
Ph. D. Ming Li
Ph. D. Tabbi Miller
Ph. D. Dominic Clarke
Ph. D. Brian Rafferty
M.S. Carrie LeSeur
M.S. Damien Tagliente
M.S. Mate Stulic
M.S. Matthew Skerrett
M.S. Kevin Horn
M.S. Adam Gerber
M.S. Eav K. Lim
M.S. Jinghui Qian
 
UNDERGRADUATE RESEARCH SUPERVISION :
Undergraduate Senior Honors Thesis Committee:
Jennifer Val (May 2001)
Jessica Wong (May 2000)
 
Undergraduate Researchers in my lab
1999-2000

Jill Pappalardi
Celeo Ramirez
Jennifer Val
Brett Dietzman
Esther Kim
Rhondye Williams
Deanna El-Sadahy

2000-2001 Jill Pappalardi
Tim Amrhein
Celeo Ramirez
Elena Bilman
Jennifer Val
Esther Kim
Lisa Wong
Marika Fraser
Yannita Nazario
2001-2002 Sabina Sandigursky
Sherin Vargese
Doug DeCandia
Rosemary Ryan
2002-2003

Rosemary Ryan
Dave Kiamanesh
Marika Fraser
Rosemary Ryan
Marika Fraser
Dave Kiamanesh
Ann Nolan
Bridgette Corsa

2004-2005 Dave Kiamanesh
Bridgette Corsa
Menelva Stanton
Ann Nolan
Evan Conte
Keya O’Brien

TEACHING EXPERIENCE :

Binghamton University
Molecular genetics (Biol 301/501; Bchm 301) Fall 2001, 4 credits- 123 students
Spring 2002, 4 credits- 132 students
Spring 2003, 4 credits- 141 students
Spring 2004, 4 credits- 149 students
Fall 2004, 4 credits- 147 students
Advanced molecular genetics (Biol 430) Spring 2001, 4 credits- 12 students (Team taught with Dr. Susannah Gal
Genetics of neurodegenerative disease (Biol 480/680C) Spring 2001, 2 credits- 16 students
Genetics (Biol 330) Spring 2000, 4 credits- 66 students
Fall 2000, 4 credits- 85 students
Cell cycle regulation (Biol 480/680V) Fall 1999, 2 credits- 16 students
 
During Graduate Studies
Histology (lab), General Biology, Human Anatomy and Physiology (lab), General Biology 101, 102, 113 (labs and team lecture), Insect Physiology (Graduate Level), Insect Toxicology, Introductory Entomology (lab and team lecture), Insect Physiology 631 (labs and team lecture)

PROFESSIONAL SOCIETIES:
American Association for the Advancement of Science, Entomological Society of America, Society for Developmental Biology, Society for Neuroscience

COMMUNITY SERVICE:
2002 Forensic Entomolgy Talk- NYSME, Owego, NY
2001 Cancer talk (Pittsburgh Cancer Society)
1999-2004 Bug talks (Assorted schools)
1999-2000 Insect Expo (Binghamton, NY)
1996-1997 President of the Entomological Graduate Student Association (EGSA)
1996-1997 4H insect collection judge- Ohio State Fair
1994-1997 Volunteer at Insect days- Columbus Zoo
1993-1996 Ohio State University Insectary tours.
1994-1997 Captain- Ohio State University Linnaean Games Team

RESEARCH EXPERIENCE:
1D and 2D PAGE, western blot hybridization, Northern, Southern, and Western blot hybridization, cDNA library construction and screening, in situ hybridization, polyclonal antibody construction, primary cell culture, PCR, RT-PCR (semi-quantitative), gas chromatography, genomic DNA isolation, gene cloning, DNA sequencing, HPLC, FPLC, and total and poly (A)+ RNA isolation, immunofluorescence, immunoprecipitation.

PRESENTATIONS AT SCIENTIFIC MEETINGS
(While at Binghamton University)
2004 Hilburger EW, E Conte, L Begdeche and SP Tammariello. Expression of Nox1 and Nox4 during neuronal apoptosis. Society for Free Radical Biology and Medicine Annual Meeting. St. Thomas, US Virgin Islands.
2004 Pavlides, S, K Weir and SP Tammariello Cell cycle regulation during overwintering in the flesh fly Sarcophaga crassipalpis. Life in The Cold 2004. Seward, Alaska.
2004 Molly M. Deak, Savvas Pavlides, Feilei Huang, Rosemary C. Ryan and Steven P. Tammariello. Diapause-specific hemolymph proteins in the tobacco hornworm, Manduca sexta: a possible role in cold tolerance during overwintering? Life in the Cold 2004. Seward, Alaska
2003 Rinehart JP, GD Yocum, SP Tammariello, KH Joplin, HA Miller III, and DL. Denlinger. Molecular characterization of Sarcophaga crassipalpis diapause by microarray hybridization. Entomological Society of America. Cincinnati, OH
2003 Steadman, DW., Wilson, J., Sheridan, KE, and S.P. Tammariello. Impact of heat and chemical maceration on DNA recovery and cut mark analysis. American Academy of Forensic Science. Buffalo, NY.
2002 Gene Expression During Nerve Cord development in the tobacco hornworm, Manduca sexta. Fourth
International Symposium on Molecular Insect Science. Tucson, Arizona.
2001 Yeager-Hall, C. and S.P. Tammariello. Molecular regulation of nerve cord development in the tobacco hornworm, Manduca sexta. International Workshop on Molecular Biology and Genetics of the Lepidoptera. Kolympari, Crete, Greece
Tammariello, S.P., K.S. Snyder, J. Bu, R. Doonan, M.T. Quinn, and S. Estus. NADPH oxidase and neuronal apoptosis. Society for Neuroscience. New Orleans, Louisiana.
2000 Tammariello, S.P. Cell Cycle regulation during insect diapause. International Congress of Entomology. Iguassu Falls, Brazil.
Tammmariello, S.P. and K.S. Snyder. NADPH oxidase and neuronal apoptosis. Oxygen Society Annual Meeting. San Diego, California
Tammariello, S.P. and S. Estus. Is NADPH oxidase required for neuronal apoptosis? Oxidative Energy/ Metabolism Conference. Rockerfeller University. New York, New York.
Tammariello, S.P., M. Quinn, and S. Estus. Early critical events in NGF-deprived sympathetic neuronal apoptosis. Society for Neuroscience. Miami, Florida


(While at other Universities)
1998 Tammariello, S.P. and D.L. Denlinger. Cell cycle regulation during diapause in the flesh fly, Sarcophaga crassipalpis. International Insect Molecular Biology Symposium. Snowbird, Utah.
1998 Tammariello, S.P., M. Quinn, and S. Estus. The role of NADPH oxidase during programmed cell death in sympathetic neurons. Symposium on the Biology of Aging. Lexington, Kentucky.
1997 Tammariello, S.P. and D.L. Denlinger. Expression and function of PCNA during cell arrest in diapausing flesh flies. Entomological Society of America North Central Branch Meeting. Columbus, Ohio.
1997 Stewart, R.L., S.P. Tammariello, and G.R. Needham. Overwintering biology of Amblyomma americanum and Dermacentor variabilis. Entomological Society of America North Central Branch Meeting. Columbus, Ohio.
1997 Tammariello, S.P., G.D. Yocum and D.L. Denlinger. Isolation of diapause-associated clones from the flesh fly, Sarcophaga crassipalpis. Entomological Society of America. Nashville, Tennessee.
1996 Stewart, R.L., S.P. Tammariello, M. Selim, and G. Needham. Overwintering survivorship of two Ixodid ticks in Ohio. Entomological Society of America North Central Branch Meeting. Omaha, Nebraska.
1996 Tammariello, S.P. and D.L. Denlinger. G0/G1 cell cycle arrest in brain cells from diapausing pupae of the flesh fly, Sarcophaga crassipalpis. Midwest Regional Meeting of the Society for Developmental Biology. Chicago, Illinois.
1996 Tammariello, S.P. and D.L. Denlinger. Expression of proliferating cell nuclear antigen (PCNA) during the G0/G1 cell cycle arrest in diapausing flesh flies. Entomological Society of America National Meeting. Louisville, Kentucky.
1996 Stewart, R.L., S.P. Tammariello, and G.R. Needham. Caution tick crossing: movement of Dermacentor variabilis near roads. Entomological Society of America National Meeting. Louisville, Kentucky.
1995 Tammariello, S.P. and D. L. Denlinger. G1 cell cycle arrest exhibited in brain cells from diapausing flesh fly, Sarcophaga crassipalpis. Entomological Society of America National Meeting. Las Vegas, Nevada.

INVITED TALKS (Universities and Scientific Meetings):
2003 Live and Let Diapause. West Virginia University. Morgantown, West Virginia.
2002 Intracellular Signaling in insect dormancy. Ohio University. Athens, Ohio
2002 ROS generation in neuronal PCD. Department of Biological Sciences. Kent State University. Kent, Ohio
2001 Oxidative stress in neurodegenerative disease. Marywood University. Scranton, Pennsylvania.
2001 Reactive oxygen species generation in Alzheimer's Disease. Millersville University. Millersville, Pennsylvania.
2001 Molecular control of insect dormancy. Department of Biological Sciences. Duquesne University. Pittsburgh, Pennsylvania.
2000 The role of NADPH oxidase in Alzheimer's Disease. Department of Biological Sciences. Syracuse University. Syracuse, New York.
2000 The role of oxidative stress in neuronal apoptosis. Guthrie Institute. Sayre, Pennsylvania
1997 A subtractive library strategy for the isolation of diapause-specific clones. Sanders-Brown Center on Aging. University of Kentucky Medical Center.
1997 Live and let Diapause. Department of Pediatrics. Massachusetts General Hospital. Boston, Massachusetts.

IN-HOUSE PRESENTATIONS
2004 Pavlides S. and S.P. Tammariello. PCNA expression during insect diapause. Biology Research Symposium (BU).
DiAntonio L. and S.P. Tammariello. DNA extraction efficiency from multiple maceration techniques on pig bone. Biology Research Symposium (BU)
2004 Weir, K and S.P. Tammariello. RNAi in the flesh fly, Sarcophaga crassipalpis. Biology Research Symposium (BU)
Hilburger, E. and S.P. Tammariello. Oxidative Stress in Cellular Apoptosis. Biology Research Symposium (BU).
DeCandia, D. and S.P. Tammariello. Temporal and spatial expression of dalp during tobacco hornworm development. Biology Research Symposium (BU)
Yuan, J and S.P. Tammariello. Differential gene expression during nerve cord development in the tobacco hornworm. Biology Research Symposium (BU).
Pavlides, S and S.P. Tammariello. Gene expression during the diapause program in the flesh fly, Sarcophaga crassipalpis. Biology Research Symposium (BU)
DiAntonio, L. and S.P. Tammariello. Use of RAPDs for the identification of forensically important Dipteran species. Biology Research Symposium (BU).
2002 Sheridan, K. and S.P. Tammariello. Isolation of Mycobacterium tuberculosis DNA from an historic almshouse skeletal sample. Biology Research Symposium (BU).
2001 Snyder, K.K., R. Garruto and S.P. Tammariello. Protein kinase activity associated with Guamanian Parkinsonism. Clinical Campus Poster Session. Binghamton/Syracuse Universities.
2001 Yeager-Hall, C., C. Ramirez and S.P. Tammariello. Differential gene expression during nerve cord development in the tobacco hornworm, Manduca sexta Biology Research Symposium (BU).
2000 Yeager-Hall, C. and S.P. Tammariello. Molecular regulation of insect dormancy. Biology Research Symposium (BU)
1999 Tammariello, S.P. Live and Let Diapause: The role of cell cycle regulation during insect hibernation. Biology Research Symposium, (BU)

PUBLICATIONS:
2004 Deak, T., K.A. Bordner, N.K. McElderry, C.J. Barnum, P. Blandino, M.M. Deak and S.P. Tammariello. Stress-induced increases in hypothalamic IL-1: A systematic analysis of multiple stressor paradigms. (Brain Research Bulletin- in press)
2004 Hayward, S.A., S. Pavlides, J. Reinhart, S.P. Tammariello, and D.L. Denlinger. Temporal expression patterns of diapause-associated genes in flesh fly pupae from the onset of diapause through diapause quiescence. (Journal of Insect Physiology- in press)
2004 Hilburger E., E. Conte, D.W. McGee and S.P. Tammariello. Localization of NADPH oxidase subunits in neonatal sympathetic neurons. (Neuroscience Letters- in press)
Hilburger E. and S.P. Tammariello. Angiotensin II accelerates apoptosis following NGF withdrawal in sympathetic neurons. (Submitted to Molecular Brain Research)
2004 Steadman D.W., L. A. DiAntonio, J. J. Wilson, K.E. Sheridan and S.P. Tammariello. The Effects of Chemical and Heat Maceration Techniques on the Recovery of Genomic and Mitochondrial DNA from Bone. (Submitted to Journal of Forensic Sciences).
2004 Hilburger E., K. O’Brien, C. Ross and S.P. Tammariello. The synthetic peptide PR-39 inhibits apoptosis in NGF-deprived sympathetic neurons. (Submitted to NeuroReport)
2004 Pavlides, S, K. Weir and S.P. Tammariello. Live and Let Diapause: Cell Cycle Regulation During Insect Overwintering. In: Life in the Cold. Evolution, Mechanisms, Adaptation and Application. Institute of Arctic Biology. 293-298. (Book Chapter)
2001 Bruckner SR, SP Tammariello, CY Kuan, RA Flavell, P Rakic,and S Estus. JNK3 contributes to c-Jun activation and apoptosis but not oxidative stress in nerve growth factor-deprived sympathetic neurons. J Neurochem 78(2):298-303
2001 Tammariello, S.P. Regulation of the cell cycle during diapause. In: Insect Timing: Circadian Rhythmicity to Seasonality. Elsevier Scientific. 173-184. (Book Chapter)
2001 Tammariello, S.P., G. E. Landreth, and S.Estus. The role of Jun-Kinases in Apoptosis. In: Programmed Cell Death. Volume I. Elsevier Scientific. 197-214. (Book Chapter)
2000 Tammariello, S.P. , M. Quinn, and S. Estus. The role of NADPH oxidase in NGF deprivation-induced neuronal apoptosis. J. Neurosci. 20: RC53.
2000 P. Nair, S.P. Tammariello and S. Estus. Genetic and metabolic status of ceramide- saved, NGF-deprived sympathetic neurons. Cell Death Differ. 7(2):207-214.
1999 Chan SL, SP Tammariello, S Estus, and MP Mattson. Prostate apoptosis response-4 mediates trophic factor withdrawal-induced apoptosis of hippocampal neurons: actions prior to mitochondrial dysfunction and caspase activation. J Neurochem 73(2):502-12.
1999 Tammariello, S.P., J.P. Rinehart, and D.L. Denlinger. Desiccation elicits heat shock transcription in the flesh fly, Sarcophaga crassipalpis, but does not enhance tolerance to high and low temperatures. Journal of Insect Physiology. 45: 933-938.
Tammariello, S.P. and Denlinger, D.L. Expression of PCNA during the G0/G1 cell cycle arrest in diapausing flesh flies. Insect Biochemistry and Molecular Biology. 28(2); 83-89.
1998 Flannagan, R.D., S.P. Tammariello, K.H. Joplin, R.A. Cikra-Ireland, G.D. Yocum and D.L. Denlinger. Gene expression during diapause in the flesh fly Sarcophaga crassipalpis. Proceedings of the National Academy of Sciences (USA). 95(10); 5616- 5620.
1998 Tammariello, S.P. and D.L. Denlinger. Cloning and sequencing of PCNA from the flesh fly, Sarcophaga crassipalpis, and its expression during cold shock and heat shock. Gene. 215(2); 425-429.
Stewart, R.L., S.P. Tammariello, D.M. Yehling, and G.R. Needham. Comparative overwintering survival of Amblyomma americanum and Dermacentor variabilis in Georgia and Ohio. International Journal of Acarology. 24(1); 81-85.
Denlinger, D.L., K.H. Joplin, R.D. Flannagan, S.P. Tammariello, M-L. Zhang,G.D. Yocum, and K.-Y. Lee. Diapause-specific gene expression. In: Molecular mechanisms of insect metamorphosis and diapause. A. Suzuki, H. Kataoka, and S. Matsumoto, Industrial Publishing & Consulting, Inc., Tokyo, pp289-297.

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Email : tammarie@binghamton.edu