Brian Callahan

Dr. Brian P. Callahan

Assistant Professor of Biological Chemistry

  • B.S., State University of New York, Cortland, 1996
  • Ph.D., University of North Carolina, Chapel Hill, 2005
Science 2 Bldg - Room 326
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Research Interests:

We investigate the chemical biology of protein biogenesis and protein degradation, two essential events in the expression of genetic information. Our research in this area is technology and hypothesis driven, with applications to major human diseases such as tuberculosis and cancer.

Recent Selected Publications

Owen, T.S.; Ngoje, G.; Lageman, T.J.; Bordeau, B.M.; Belfort M.; Callahan B.P. Förster Resonance Energy Transfer-based Cholesterolysis Assay Identifies a Novel Hedgehog Inhibitor. Anal. Biochem. 2015 (in press)

Xie J.; Owen T.; Xia K.; Singh A.V.; Tou E.; Li L.; Arduini B.; Li H.; Wan L.Q.; Callahan B.; Wang C.  Zinc Inhibits Hedgehog Autoprocessing: Linking Zinc Deficiency with Hedgehog Activation. J. Biol. Chem. 2015, 290, 11591-600.

Owen, T.S.; Xie, X.J.; Laraway B.; Ngoje G.; Wang C.; Callahan B.P. Active site targeting of hedgehog precursor protein with phenylarsine oxide. Chembiochem. 2015, 16, 55-8.

Callahan B.P.; Belfort M. Branching out of the intein active site in protein splicing. Proc. Nat'l. Acad. Sci. 2014, 111, 8323-4.

Callahan B.P.; Stanger M.; Belfort M. A redox trap to augment the intein toolbox. Biotechnol Bioeng. 2013, 110, 1565-73.

Xie J.; Du Z.; Callahan B.; Belfort M.; Wang C. (1)H, (13)C, and (15)N NMR assignments of a Drosophila Hedgehog autoprocessing domain. Biomol NMR Assign. 2013, Jun 14.

Dearden A.K.; Callahan B.; Roey P.V.; Li Z.; Kumar U.; Belfort M.; Nayak S.K. A conserved threonine spring-loads precursor for intein splicing. Protein Sci. 2013, May 22(5), 557-63.

Callahan B.P.; Topilina, N.; Stanger M.; Van Roey P.; Belfort M. Structure of a catalytically competent intein caught in a redox trap with functional and biological implications. Nat. Struct. Mol. Biol. 2011, 18, 630-3.

Callahan B.P.; Stanger M.; Belfort M. Protease activation of split green fluorescent protein. ChemBioChem 2010, 11, 2259-63.

Amitai G.; Callahan B.P.; Stanger M.; Belfort G.; Belfort M. Modulation of intein activity by its neighboring extein substrates. Proc. Natl. Acad. Sci. USA 2009, 106, 11005-10.

Callahan B.P.; Yaun, Y.; Wolfenden R. The burden borne by urease. J. Am. Chem. Soc. 2005, 127, 10828-9. (Highlighted in Science)

Last Updated: 8/12/15