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A Closer Look at
Dave Werner's Research
DEARC Call for Pilot Proposals
Letter of Intent due: January 15
Proposals due: February 15
Research for Society on Alcoholism
38th Annual Meeting
June 20-24, 2015
San Antonio, Texas
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A primary goal of the DEARC is to elucidate the brain and behavioral consequences of ethanol exposure throughout a broad range of developmental stages encompassing gestation through adulthood. The majority of projects described in this center proposal utilize rodent models, making the Animal/Behavior Core a critical component of the DEARC. The Animal/Behavior Core (ABC) plays several key roles within the center: It provides guidance and training on breeding practices, ethanol exposure, surgical techniques and behavioral phenotyping. In addition, the ABC provides access to equipment for behavioral assessment and measurement of ethanol in blood and brain. Another major goal of the ABC is to facilitate the sharing of animal resources through an interactive on-line database. Specifically, the ABC will streamline the generation, use, and distribution of animal resources, while at the same time providing crucial support for the standardization of techniques, thereby assuring quality control. Finally, the ABC is critical in maintaining the overarching goal of the DEARC of cross-study comparisons to understand how developmental ethanol exposure changes brain and behavioral function across the lifespan.
The NeuroCore provides DEARC investigators with access to state-of-the-art resources and equipment for conducting molecular, cellular, or neuroanatomical related research. These studies involve quantification, visualization, or manipulation of RNA, DNA, or protein. At every stage of investigation, the NeuroCore personnel will ensure the highest quality of data is established and maintained, that uniform techniques are applied across projects to maximize data harmonization, and that tissue resources used by investigators are preserved as much as possible for future studies. NeuroCore personnel will also regularly meet with individual DEARC investigators to help plan specific details of their experiments and assist in data analysis and training of Project or Pilot personnel. To accomplish these goals, the NeuroCore is designed to consist of three complimentary components, each one directed by a separate investigator. First, a Molecular Component provides laser microdissection, nucleic acid and protein purification and several quantitative measures of gene or protein expression. Second, a Neuroanatomy Component provides immunoflourescent and immunohistochemical techniques to map regional alterations in neuronal and glial phenotypes and changes or adaptations in cells or circuity due to alcohol exposure. Third, a Cellular Component provides the resources and expertise for conducting in vitro experiments in primary neuronal, glial and cell line specific cultures, helps investigators construct and validate appropriate vectors for gene delivery or knockdown experiments, and enables future studies that might involve genetically modified rodent models.