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Techniques

Rodent Models and Surgical Techniques

  • Stereotaxic surgery in rat (brain cannulations,  drug microinjections and lesions)
  • Brain dissections in rat (hippocampus, hypothalamus, cortex, cerebellum, pituitary, nucleus tractus solitarius/area postrema, ventral tegmental area, nucleus accumbens, etc)
  • Adrenalectomy and ovariectomy in rat
  • Cecal ligation and puncture (animal model of infection)
  • Thermister implantation and core body temperature measurement
  • Basic histology, light microscopy and image analysis (NIH Image, Un-scan-it, Imagegauge)
  • Behavioral models:  social play/interaction testing, conditioned fear, escape behavior, elevated plus maze, forced swim test
  • Developed two distinct models of subcutaneous inflammation/infection to study wound healing

 

Biochemical and Molecular assays

  • Competitive binding assay for corticosteroid binding globulin (CBG)
  • Receptor binding assay for corticosteroid receptors (MR & GR)Experion
  • Scatchard analysis
  • Bradford & Lowry protein assays
  • Seromucoid protein assay (a1-acid glycoprotein)
  • Radioimmunoassays for corticosterone, adrenocorticotrophic hormone (ACTH), and testosterone
  • Western blotting (glucocorticoid receptors, mineralocorticoid receptors, CRH receptors, IL-1, pro-IL-1, CD45, CD14, MHCII, opioid ligands and their receptors, etc.)
  • Enzyme linked immunosorbent assays (ELISAs;  IL-1,  IL-6,  IL-10,  BDNF, etc.)
  • Bacterial culture (Escherichia coli & Streptococcus mutans)
  • Reverse Transcription - Polymerized Chain Reaction (RT-PCR) – in real time and classic version where PCR product is resolved by gel electrophoresis;  includes all steps leading up to RT-PCR as well (RNA extraction & purification, RNA quantification, first strand synthesis, etc.)
  • In situ hybridization

Last Updated: 11/5/13