Research Techniques and Protocols
- Aseptic technique
- Viable plate counting
- Optical density and absorbance measurements
- Continuous-culture biofilm growth
- Microscopy (static and flow-cell)
- Biofilm assays (antibiotic susceptibility, dispersion, biomass)
- SDS protein gels
- Polymerase chain reaction techniques (PCR, qRT-PCR)
- Bacterial molecular cloning
- Basic biological statistics
Lab Equipment
- Incubators
- Laminar flow hood
- Bench-top autoclaves
- Centrifuges
- Spectrophotometers (cuvette and plate)
- Olympus Brightfield and fluorescent microscopes
- Gel doc camera
- Peristaltic pumps for continuous-culture biofilm growth
- PCR thermocyclers
- Proteomic and western blotting apparatuses
Key Peer-Reviewed Publications
- Sauer K, Camper AK, Ehrlich GD, Costerton JW, Davies DG. 2002. Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm. J Bacteriol 184:1140–1154.
- Davies DG, Marques CNH. 2009. A fatty acid messenger is responsible for inducing dispersion in microbial biofilms. J Bacteriol 191:1393–403.
- Florez C, Raab JE, Cooke AC, Schertzer JW. 2017. Membrane distribution of the Pseudomonas quinolone signal modulates outer membrane vesicle production in Pseudomonas aeruginosa. MBio.
- Woods PW, Haynes ZM, Mina EG, Marques CNH. 2019. Maintenance of S. aureus in Co-culture With P. aeruginosa While growing as biofilms. Front Microbiol.
- Cook LCC, Hu H, Maienschein-Cline M, Federle MJ. 2018. A vaginal tract signal detected by the group b streptococcus saers system elicits transcriptomic changes and enhances murine colonization. Infect Immun.
Additional Resources
National
- American Society for Microbiology
- American Society for Biochemistry and Molecular Biology
- Federation of European Microbiological Societies
Local
