Biofilms Lab

Research Techniques and Protocols

  • Aseptic technique
  • Viable plate counting
  • Optical density and absorbance measurements
  • Continuous-culture biofilm growth
  • Microscopy (static and flow-cell)
  • Biofilm assays (antibiotic susceptibility, dispersion, biomass)
  • SDS protein gels
  • Polymerase chain reaction techniques (PCR, qRT-PCR)
  • Bacterial molecular cloning
  • Basic biological statistics

Lab Equipment

  • Incubators
  • Laminar flow hood
  • Bench-top autoclaves
  • Centrifuges
  • Spectrophotometers (cuvette and plate)
  • Olympus Brightfield and fluorescent microscopes
  • Gel doc camera
  • Peristaltic pumps for continuous-culture biofilm growth
  • PCR thermocyclers
  • Proteomic and western blotting apparatuses

Key Peer-Reviewed Publications 

Additional Resources